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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Effect of Posttranslational Modifications on the Structure and Activity of FTO Demethylase
doi: 10.3390/ijms22094512
Figure Lengend Snippet: Compositions of buffers.
Article Snippet: DSF6 , Nano Differential Scanning Fluorimetry , Evaluation effect of dephosphorylation on EC FTO and BES FTO thermal stability , 37 mM HEPES, 1mM MES, pH 7.5, 124 mM NaCl, 37% ( v/v ) glycerol, 0.01% Brij 35, 3.7 mM β-mercaptoethanol, 2 mM DTT, 1 mM 2-OG, 1 mM Mn Cl 2 , 800 U of
Techniques: Mutagenesis, Transformation Assay, Protein Purification, Lysis, Nano Differential Scanning Fluorimetry, De-Phosphorylation Assay, Filtration, Chromatography, Microscale Thermophoresis